Plant Tissue Genomic DNA Isolation Kit

Plants serve a vital role in human nutrition, biofuels, and the ecosystem. Isolating DNA from plants is exceptionally challenging compared to other organisms due to the presence of difficult to disrupt cell wall and compounds such as tannins, phenolics, and complex polysaccharides that can affect DNA quality and inhibit downstream reactions. BioVision’s Plant Genomic DNA Isolation kit enables the researchers to isolate genomic DNA from plant leaves, stalks, seeds, and roots. This kit utilizes an efficient combination of lysis buffer and enzymes that work in unison to disrupt the cell wall, eliminate plant specific contaminating compounds, and release free genomic DNA. DNA release from the cell is coupled with adsorption of DNA onto a silica spin-column under chaotropic conditions, eliminating the use of toxic organic compounds or solvents. This kit yields pure DNA that is suitable for various downstream molecular biology applications such as PCR, cloning, sequencing, DNA hybridization and Southern Blotting, etc.

• Useful for PCR, Cloning, DNA hybridization, Southern Blotting

Simple & rapid method to isolate highly pure, intact DNA from plant tissues

Plant

none

• Buffer L [Lysis Buffer] • 1 M DTT • Enzyme Mix • RNAse A • Buffer B [Binding Buffer] • Buffer W [Wash Buffer] • Buffer E [Elution Buffer] • Spin Columns/Collection Tubes

-20°C/RT

gel pack

24 months

Plants

Useful for PCR, Cloning, DNA hybridization, Southern Blotting

Fresh or dry leaf, root, and seed plant tissues

tissue

Plants RNA, DNA , cDNA and plant tissues are used to study plant genes.

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