PicoProbe™Acetyl-CoA Fluorometric Assay Kit

Acetyl CoA is a central molecule of metabolism. It carries acetate, used in the build-up and breakdown of larger molecules. Acetyl CoA is key in synthetic pathways leading to sesquiterpenes, precursors to cholesterol and other sterols, flavenoids and other polyketides, polyenes and long-chain fatty acids. It is the source of the acetyl group used in histone acetylation. The acetyl group is also incorporated into a variety of other molecules such as acetylcholine, melatonin, heme and TCA cycle intermediates. BioVision has developed a highly sensitive assay for determining Acetyl CoA level in a variety of biological samples. In the assay, free CoA is quenched then Acetyl CoA is converted to CoA. The CoA is reacted to form NADH which interacts with PicoProbe to generate fluorescence (Ex=535/Em=587 nm). The assay can detect 10 to 1000 pmol of Acetyl CoA (with detection limit ~0.4 µM) in a variety of samples.

• Detection method: Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The assay can detect 10 to 1000 pmol of Acetyl CoA (with detection limit ~0.4 μM) in a variety of samples.

• Very simple procedure • Convenient • BioVision has developed a high sensitivity assay for Acetyl CoA level in a variety of biological samples. In the assay, free CoA is quenched then Acetyl CoA is converted to CoA. The CoA is reacted to form NADH which interacts with PicoProbe to generate fluorescence.

Coenzyme A

none

• Acetyl CoA Assay Buffer • PicoProbe • Conversion Enzyme • Acetyl CoA Enzyme Mix • Acetyl CoA Substrate Mix • CoA Quencher • Quench Remover • Acetyl CoA Standard (10 µmol)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

N terminal acetylation or CH3CO as epigenetic regulation of ™ -CoA Fluorometric Assay Kit by NATs.

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit