HAT Activity Colorimetric Assay Kit

Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. BioVision’s HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.

• Detection method: Absorbance (440 nm) • Species reactivity: Mammalian • Applications: Detects HAT activity by colorimetric method in mammalian samples

• Simple procedure • Fast and convenient • The detection can be continuous and suitable for kinetic studies

HAT

none

• 2X HAT Assay Buffer • HAT Substrate I • HAT Substrate II • NADH Generating Enzyme • HAT Reconstitution Buffer • Nuclear Extract 4 mg/ml

-20°C

dry ice

12 months

see datasheet

Inquire

Cell and tissue lysates

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.