NAD/NADH Quantitation Colorimetric Kit

Assay of nicotinamide nucleotides is of continual interest in the studies of energy transforming and redox state of cells or tissues. BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio. The NAD Cycling Enzyme Mix in the kit specifically recognizes NADH/NAD in an enzyme cycling reaction. There is no requirement to purify NADH/NAD from samples. The reaction specifically detects NADH and NAD, but not NADP nor NADPH. The enzyme cycling reaction significantly increases the detection sensitivity and specificity. NADt (NAD and NADH) or NADH can be easily quantified by comparing with standard NADH.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio.

• Simple procedure; takes ~2 hours • Fast and convenient • Kit contains the necessary reagents for accurate measurement of NAD and NADH and their ratio.

NAD/NADH

none

• NADH/NAD Extraction Buffer • NAD Cycling Buffer • NAD Cycling Enzyme Mix • NADH Developer • Stop Solution • NADH Standard (MW:763)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Colorimetric assays or detection use UV absorption or enzymatic color reaction.