HDAC1 Immunoprecipitation (IP) & Activity Assay Kit

Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. HDAC1 is ubiquitously expressed and plays a role in cellular senescence, cardiomyocyte differentiation and hypertrophy, aging of the liver, myelination, and adult neurogenesis to name a few functions. The HDAC1 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC1 complex from cells & tissues and to measure HDAC1 activity fluorometrically. HDAC1 is immunoprecipitated from cell, nuclear or tissue extract(s) using HDAC1 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.

• Detection method : Fluorescence (Ex/Em=380/500 nm) • Applications: : Immunoprecipitation of HDAC1 complex from cell, nuclear, or tissue extract(s) : Measurement of HDAC1 activity of immunoprecipitated complex/purified enzyme : Screening for activators or inhibitors of HDAC1

• Simple, fast and convenient. • Only kit on the market for both IP as well as measuring activity of HDAC1

HDAC

Histone Deacetylase 1 Immunoprecipitation (IP) & Activity Assay Kit

• HDAC Assay Buffer • Cell Lysis Buffer • HDAC Substrate • Developer • AFC (7-amino-4-trifluoromethyl coumarin) Standard (1 mM) • Rabbit HDAC1 Antibody • Rabbit IgG (Control Antibody) • Protein-A/G Sepharose Beads (50% slurry in 20% ethanol/H2O) • Positive Control (Jurkat Cell Lysate) Lyophilized

-20°C

gel pack

12 moths

see datasheet

Inquire

Human, Mouse or Rat • Cell Lysate and Nuclear Extract • Tissue Extract

Biovision supplies other types of Assays as 1.