HDAC3 Activity Fluorometric Assay Kit

Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. HDACs are transcriptional repressors with crucial roles in mammalian development. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In BioVision’s HDAC-3 activity Kit, HDAC-3 and Developer will deacetylate and cleave the substrate [R-H-K-K(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput measurement of HDAC-3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC3 samples.

• Detection method: Fluorescence (Em/Ex = 380/500 nm) • Applications: Suitable for high throughput measurement of HDAC3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC:8 samples.

• Simple procedure; takes ~ 60 min • Fast and convenient • Kit contains the necessary reagents for accurate measurement of HDAC3 activity

HDAC

none

• HDAC3 Assay Buffer • HDAC3 Substrate • HDAC3 Positive Control • AFC Standard (1 mM) • Developer • Trichostatin A

-20°C

gel pack

12 months

see datasheet

Inquire

Purified, immunoprecipitated and recombinant or genetically modified HDAC3

Biovision supplies other types of Assays as 1.