NADP/NADPH Quantitation Colorimetric Kit

Catalog number
K347-100
Name
NADP/NADPH Quantitation Colorimetric Kit
Size
100 assays
Price
581.00 EUR
Supplier

Details

Kit's description
Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue. The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH). There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using plate reader at OD450 nm.
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: Convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio
Kit's benefits
• Simple procedure; takes ~2 hours • Fast and convenient • Kit contains the necessary reagents for accurate measurement of NADP and NADPH and their ratio • Enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH)
Category
NADP/NADPH
Kit's other name
none
Contents
• NADP/NADPH Extraction Buffer • NADP Cycling Buffer • NADP Cycling Enzyme Mix • NADPH Developer • Stop Solution • NADPH Standard (MW:833.36)
Storage condition
-70°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.