NADP/NADPH Quantitation Colorimetric Kit

Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue. The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH). There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using plate reader at OD450 nm.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: Convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio

• Simple procedure; takes ~2 hours • Fast and convenient • Kit contains the necessary reagents for accurate measurement of NADP and NADPH and their ratio • Enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH)

NADP/NADPH

none

• NADP/NADPH Extraction Buffer • NADP Cycling Buffer • NADP Cycling Enzyme Mix • NADPH Developer • Stop Solution • NADPH Standard (MW:833.36)

-70°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Colorimetric assays or detection use UV absorption or enzymatic color reaction.