HDAC8 Activity Fluorometric Assay Kit

Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. HDACs are transcriptional repressors with crucial roles in mammalian development. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In BioVision’s HDAC-8 activity Kit, HDAC-8 deacetylates the substrate (R-H-K(Ac)-K(Ac)-AFC) followed by an enzymatic cleavage to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput measurement of HDAC-8 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC-8 samples.

• Detection method: Fluorescence (Ex/Em = 380/500 nm) • Species reactivity: Mammalian • Applications: Suitable for high throughput measurement of HDAC8 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC:8 samples.

• Simple procedure; takes ~ 60 min • Fast and convenient • Kit contains the necessary reagents for accurate measurement of HDAC-8 activity

HDAC

none

• HDAC-8 Assay Buffer • HDAC-8 Substrate • HDAC-8 Positive Control • AFC Standard (1 mM) • Developer • Trichostatin A (10 μM)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue lysates, plasma and serum, other biological fluids

Biovision supplies other types of Assays as 1.