Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric)

Adenylate Kinase (AK) (EC 2.7.4.3) is an abundant enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. The enzyme is found in the nucleus, cytosol, or mitochondria (intermembrane space or matrix) of various kinds of tissues. Adenylate kinase acts on two molecules of ADP to generate ATP and AMP. Nine isoforms of adenylate kinase have been identified. Erythrocyte adenylate kinase deficiency is associated with hemolytic anemia. Adenylate kinase also plays an important role in post-ischemic recovery and in apoptosis. BioVision’s AK Activity Assay kit can kinetically measure Adenylate Kinase activity by detecting adenosine triphosphate (ATP) generated from adenosine diphosphate (ADP) as a substrate. ATP is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the Adenylate Kinase Probe forming an end product that can be measured colorimetrically (OD 570 nm) or fluorometrically (Ex/Em = 535/587 nm).

• Detection method: Absorbance (OD 570 nm) or Fluorescence (Ex/Em = 535/587 nm) • Application: Detection of Adenylate Kinase activity

• Simple, rapid & convenient • The kit includes all the reagents required to measure AK activity

Adenylate Kinase

none

• AK Assay Buffer • AK Probe • ADP Substrate • AK Convertor • AK Developer • Positive Control (AK Enzyme) • ATP Standard (1 µmol)

-20°C

gel pack

12 months

see datasheet

Detection of Adenylate Kinase activity

• Purified Adenylate Kinase • Cell and tissue lysate • Mitochondrial lysate

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.