FAD Colorimetric/Fluorometric Assay Kit

Flavin Adenine Dinucleotide (FAD) is a redox cofactor which plays an important role in metabolism. FAD exists in different redox states and cycles between FAD, FADH and FADH2. The primary sources of reduced FAD in eukaryotic metabolism are the citric acid cycle and the beta oxidation reaction pathways. In BioVision’s FAD Assay Kit, FAD functions as the cofactor of an oxidase which catalyze the formation of a product that reacts with OxiRed probe generating color and fluorescence. FAD can be detected by either colorimetric (λ=570 nm) or fluorometric (Ex/Em=535/587 nm) methods. The kit provides a rapid, simple, ultra-sensitive, and reliable test suitable for high throughput assay of FAD. The lower limit of detection is less than 1 nM FAD.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: FAD functions as the cofactor of an oxidase which catalyze the formation of a product that reacts with OxiRed probe generating color and fluorescence.The lower limit of detection is less than 1 nM FAD.

• Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable.

FAD

none

• FAD Assay Buffer • OxiRed Probe • Dimethylsulfoxide (DMSO; Dried) • FAD Enzyme Mix (lyophilized) • FAD Standard (1 nmol) • Stop Solution

-20°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.