Factor IXa Activity Assay Kit (Fluorometric)

The coagulation Factor IX (or Christmas factor) (EC 3.4.21.22) is a vitamin K-dependent serine protease. Factor IX is produced as an inactive precursor and is activated via cleavage by either factor XIa (contact pathway) or factor VIIa (tissue factor pathway). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor IX (FIXa) then binds to the activated Factor VIII (FVIIIa) and proteolytically activates factor X (FX) to factor Xa (FXa). BioVision’s Factor IXa Activity Assay kit is based on the ability of FIXa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by fluorescence microplate reader. The assay is simple, rapid and can detect activity as low as 10 pg of FIXa in a variety of samples.

• Detection method: Fluorescence (Ex/Em = 360/450 nm • Applications: : Determine activity of purified Factor IXa : Detect the activity of Factor IXa in plasma and serum samples

• Rapid and convenient • The assay can detect Factor IXa activity as low as 10 pg.

factor ixa, fixa

none

• FIXa Assay Buffer • FXa Substrate-AMC • Enzyme Mix I • Enzyme Mix II • Phospholipids • FIXa Enzyme Standard (10 ng)

-20°C

gel pack

12 months

see datasheet

• Determine activity of purified Factor IXa • Detect the activity of Factor IXa in plasma and serum samples

Purified Factor Ixa, Plasma, Serum

Biovision supplies other types of Assays as 1.

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.