PEP Colorimetric/Fluorometric Assay Kit

Phosphoenolpyruvate (PEP) is an important intermediate in carbohydrate metabolism. Containing a high-energy phosphate bond, PEP is involved in glycolysis and gluco-neogenesis and in the shikimate pathway as well as in carbon fixation in plants. Bacteria utilize PEP in the phosphor-transferase system to acquire sugars from the environment. In the glycolytic pathway, PEP is formed from 2-phosphoglycerate by enolase and generates ATP through the action of pyruvate kinase. BioVision’s PEP Assay Kit provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples. In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (λmax = 570 nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, sensitive and reliable. The detection limit is approximately 1 µM PEP in biological samples.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The detection limit is approximately 1 µM PEP in biological samples.

• Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable.

PEP

none

• PEP Assay Buffer • PEP Probe (in DMSO) • PEP Converter • PEP Developer Mix • PEP Standard (1 μmole)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue lysates, urine, plasma, serum, and plant extracts

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.