TACE Inhibitor Screening Assay Kit ( Fluorometric)

The TACE (tumor necrosis factor-α-converting enzyme), also called ADAM metallopeptidase domain 17 (ADAM17), is a 70-kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. TACE is believed to be involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. This process, which is also known as 'shedding', involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-α), and is of known physiological importance. In BioVision’s TACE inhibitor screening Kit, TACE hydrolyzes the specific FRET substrate to release the quenched fluorescent group, which can be detected fluorometrically at Ex/Em = 318/449 nm. In the presence of the potent TACE inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive and reliable test suitable for high-throughput screening of TACE inhibitors and can be modified to check the relative TACE activity. Inhibitor Control GM6001 is included to compare the efficacy of test inhibitors.

• Detection method: Fluorescence (Ex/Em 318/449 nm) • Species reactivity: Mammalian • Applications: Provides a rapid, simple, sensitive and reliable test suitable for high:throughput screening of TACE inhibitors and can be modified to check the relative TACE activity. Inhibitor control GM6001 is included to compare the efficiency of test inhibitors.

• Simple procedure • Fast and convenient • The assay is sensitive, simple, and high-throughput adaptable.

TACE

none

• TACE Assay Buffer • TACE Substrate • TACE Enzyme (20 μg) • Inhibitor Control (0.1 mM GM6001)

-20°C

gel pack

12 months

see datasheet

Inquire

N/A

Biovision supplies other types of Assays as 1.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.