cAMP Direct Immunoassay Kit (Colorimetric)

Adenosine 3’,5’-cyclic monophosphate (cyclic AMP, cAMP) is an important “second messenger” involved in many physiological processes. BioVision’s cAMP Assay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cAMP level in biological samples. The kit utilizes recombinant Protein G coated 96-well plate to efficiently anchor cAMP polyclonal antibody on to the plate. cAMP-HRP conjugate directly competes with cAMP from sample binding to the cAMP antibody on the plate. After incubation and washing, the amount of cAMP-HRP bound to the plate can easily be determined by reading HRP activity at OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cAMP in samples. In addition, the kit provides a new acetylation procedure to improve detection sensitivity significantly. The kit can detect ~0.1-10 pmol/5 μl (or ~ 0.02-2 μM) cAMP samples.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: The kit can detect ~0.1:10 pmol/5 μl (or ~ 0.02:2 μM) cAMP samples.

• Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • The kit provides a new acetylation procedure to improve detection sensitivity significantly

cAMP

none

• 10X cAMP Assay Buffer • Standard cAMP (10 nmol) • Neutralizing Buffer • Acetylating Reagent A • Acetylating Reagent B • Rabbit Anti-cAMP pAb • HRP Developer • Protein G Coated Plate

-20°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Colorimetric assays or detection use UV absorption or enzymatic color reaction.