cGMP Direct Immunoassay Kit (Colorimetric)

Adenosine and guanosine 3’,5’-cyclic monophosphate (cAMP and cGMP) are important “second messengers” involved in many physiological processes. BioVision’s cGMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples. The kit utilizes the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cGMP in samples. The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: The kit can detect 0.04 :10 pmol/well (0.008 : 2 µM) cGMP samples.

• Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • The kit provides a new acetylation procedure to improve detection sensitivity significantly

cGMP

none

• 10X cGMP Assay Buffer • Standard cGMP (10 nmol) • Neutralizing Buffer • Acetylating Reagent A • Acetylating Reagent B • Anti-cGMP pAb/BSA • HRP Developer • Protein G Coated Plate

-20°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Colorimetric assays or detection use UV absorption or enzymatic color reaction.