Urease Activity Assay Kit (Colorimetric)

Urease (EC 3.5.1.5) catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in certain bacteria, yeast, fungi, and plants. In addition, it is found environmentally in soil samples and fecal matter. Due to ammonia production, urease can play a role in the pathogenesis of certain medical conditions including urinary stones, peptic ulcers, and hepatic encephalopathy. As such, urease can be used as a diagnostic for gastrointestinal and urinary tract pathogens. BioVision’s Urease Activity Assay Kit provides a quick and easy way to determine urease activity. The kit uses a modified Berthelot method to detect ammonia, which can be measured at 670 nm. The kit is suitable for measuring urease activity of biological and soil samples as well as of purified enzyme. The limit of detection is below 0.001 mU.

• Detection method: Absorbance (670 nm) • Application: : Measurement of urease activity

• Simple, fast and convenient • Suitable for measuring urease activity of biological and soil samples as well as of purified enzyme • limit of detection is below 0.001 mU

Metabolism, Urease

none

• Urease Assay Buffer • Ammonia Reagent 1 • Ammonia Reagent 2 • Urea (50x) • Urease • Ammonium Chloride Standard (100 mM)

-20°C

gel pack

12 months

see datasheet

Measurement of urease activity

• Purified enzyme • Biological samples such as cells, feces, urine etc. • Soil samples

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.