Magnesium Colorimetric Assay Kit

Magnesium is the 11th most abundant element by mass in the human body. Mg²ᶧ is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Mg²ᶧ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Mg²ᶧ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia. The BioVision Magnesium Assay Kit provides a simple sensitive means of quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg²ᶧ. An enzyme linked reaction leads to formation of an intensely colored (λmax = 450nm) product whose formation is proportional to Mg²ᶧ concentration. The linear range of the assay is 2-15 nmoles with detection sensitivity~ 40 µM.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: The linear range of the assay is 2:15 nmoles with detection sensitivity~ 40 µM.

• Simple procedure; takes less ~30 minutes • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • The kit takes advantage of the specific requirement of glycerol kinase for Mg²ᶧ

none

• Magnesium Assay Buffer • Magnesium Developer • Magnesium Enzyme Mix • Magnesium Standard (150 nmol/µl)

+4°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.