Iron Colorimetric Assay Kit

Iron is essential to nearly all known organisms. It is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Inorganic iron also contributes to redox reactions in the iron-sulfur clusters of many enzymes, such as nitrogenase and hydrogenase. BioVision's Iron Assay Kit provides a simple convenient means of measuring Ferrous and/or Ferric ion in sample. In the assay, ferric carrier protein will dissociate ferric into solution in the presence of acid buffer. After reduction to the ferrous form (Fe²ᶧ), iron reacts with Ferene S to produce a stable colored complex and give absorbance at 593 nm. A specific chelate chemical is included in the buffer to block copper ion (Cu²ᶧ) interference. The kit measures iron in the linear range of 0.4 to 20 nmol in 50 µl sample, or 8 µM to 400 µM iron concentration in various samples.

• Detection method: Absorbance (593 nm) • Species reactivity: Mammalian • Applications: The kit can detect 8 µM to 400 µM iron concentration in various samples.

• Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • A specific chelate chemical is included in the buffer to block copper ion (Cu²ᶧ) interference.

Iron Assay

none

• Iron Assay Buffer • Iron Probe • Iron Reducer • Iron Standard (100 mM)

+4°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue lysates, culture media, urine, and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.