Ready-to-use IHC kit (Biotin free), One-Step HRP Polymer anti-Mouse, Rat and Rabbit IgG (H+L) with DAB

Immunohistochemistry (IHC)/Immunocytochemistry (ICC) is the localization of antigens in tissue sections/cells by the use of labeled antibodies as specific reagents through antigen-antibody interactions that are visualized by a marker such as fluorescent dye, enzyme, radioactive element or colloidal gold. Several IHC techniques are commonly used such as labeled biotin secondary antibody streptavidin-peroxidase, HRP anti-HRP, ABC catalyzed signal amplification, polymer system (one or two steps) etc. to detect antigens in tissues and cells. BioVision’s Ready-to-use IHC kit employs polymer technology that provides increased sensitivity and detection. IBSC One-Step anti-mouse, Rat and Rabbit IgG (H+L) is a biotin/avidin free system that stains membrane, cytoplasmic and nuclear antigens. It provides the user with a rapid and easy to use IHC detection system.

• Species reactivity: Mouse, rat, and rabbit • Applications: A biotin/avidin free system that stains membrane, cytoplasmic and nuclear antigens. Immunohistochemistry (IHC) and immunocytochemistry (ICC).

• Simple procedure, sensitive • Kit provides a rapid and easy to use IHC detection system

IHC/ICC Assay

none

• Ready-to-use, peroxidase block, hydrogen peroxide • Ready-to-use protein blocking solutions • Primary antibody dilution buffer • IBSC-one-step HRP-anti-mouse, rat, and rabbit polymer • Reagent BS (ready to use buffer & substrate) • Reagent C (conc. DAB chromogen)

2-8°C

gel pack

12 months

see datasheet

Inquire

Frozen and paraffin tissue sections, cell smears

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Biotinylated,HRP Conjugated

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

IgG

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus,Oryctolagus cuniculus,Rattus norvegicus