Acid Phosphatase Activity Colorimetric Assay Kit

Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. BioVision’s Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax = 405 nm) when dephosphorylated by AP. The kit can detect µU acid phosphatase activity in samples.

• Detection method: Absorbance (405 nm) • Species reactivity: Mammalian • Applications: Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.

• The assay detects acid phosphatase (AP) activity conveniently in various biological samples

Phosphate assay

none

• AP Assay Buffer • pNPP Substrate (10 TAB) • AP Enzyme • Stop Solution

-20°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.