Ammonia Colorimetric Assay Kit II

Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance. BioVision provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In this non-enzymatic assay, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (OD670 nm) using a plate reader. The kit can detect less than 1 nmol (~10 μM) ammonia using either a 96 well or 384 well microwell plate.

• Detection method: Absorbance (670 nm) • Species reactivity: Mammalian • Applications: The kit can detect less than 1 nmol (~10 μM) ammonia using either a 96 well or 384 well microwell plate.

• Simple procedure; takes ~ 30 minutes • Fast and convenient, stable and high-throughput adaptable • In this non-enzymatic assay, ammonia is used to form indophenol, a highly colored, easily quantifiable product

Ammonia

none

• Ammonia Reagent 1 • Ammonia Reagent 2 • Ammonium Chloride Standard (100 mM)

+4°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.