Adiponectin (human) ELISA Assay Kit

Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This human Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in human serum, plasma or various tissue or cell culture supernatants. In the assay, monoclonal antibody specific for human adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-human adiponectin polyclonal antibody. With HRP conjugated anti-rabbit IgG and a HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: This human Adiponectin ELISA Kit is an enzyme:linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in human serum, plasma or various tissue or cell culture supernatants

• Simple procedure • Fast and convenient

Adiponectin

AdipoQ (human) ELISA Assay Kit, Acrp30 (human) ELISA Assay Kit, apM1 (human) ELISA Assay Kit, GMP-28 (human) ELISA Assay Kit

• Antibody coated 96-well plate (12 x 8-well strips) • 10X Wash concentrate, 50 ml • 5X Diluent, 50 ml • Secondary antibody, 12 ml • 100X Detector, 150 µl • Standard, recombinant human adiponectin (64 ng), 1 vial, lyophilized • QC sample= positive control having 7-11 µg/ml human adiponectin, 1 vial, lyophilized • Substrate I, 6 ml • Substrate II, 6 ml • Stop solution, 12 ml

+4°C

gel pack

12 months

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.