Adiponectin (mouse) Elisa Assay Kit

Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This Mouse Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in mouse serum, plasma or various tissue or cell culture supernatants. In the assay, monoclonal antibody specific for mouse adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-mouse adiponectin polyclonal antibody. With HRP conjugated anti-rabbit IgG and a HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: This mouse Adiponectin ELISA Kit is an enzyme:linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in mouse serum, plasma or various tissue or cell culture supernatants

• Simple procedure • Fast and convenient

Adiponectin

none

• Adiponectin antibody-coated plate, 6x16 wells Wash concentrate (10X), 2 x 30 ml Diluent (10X), 2 x 30 ml Secondary Antibody (200X), 60 µl Detector (100X), 150 µl Mouse adiponectin Standard (lyophilized), 16 ng TMB, 12 ml Stop Solution, 12 ml Plate sealers, 2

+4°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Mus musculus