Live/Dead Cell Viability Assay Kit (for Mammalian Cells)

Catalog number
K502-100
Name
Live/Dead Cell Viability Assay Kit (for Mammalian Cells)
Size
100 assays
Price
429.00 EUR
Supplier

Details

Kit's description
Quantification of number of live and dead cells is an indispensable tool in cell biology research. BioVision’s Live/Dead Cell Viability Assay Kit, provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes. Live cell dye easily penetrates intact, live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm which can be measured at Ex/Em = 485/530 nm. Dead cell dye enters damaged cell membranes and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence (Ex/Em = 495/635 nm) in dead cells. This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.
Highlights
• Detection method: Fluorescent microscopy or FACS analysis (Ex/Em 485/530 nm) to detect stained live cells; (Ex/Em 495/635) to detect stained dead cells • Species reactivity: Mammalian • Applications: Stained live and dead cells can easily be visualized by fluorescence microscopy or FACS analysis.
Kit's benefits
Easy to use Protocol Non-radioactive detection. Detect using either microscopy or FACS. Measure Cell Proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis
Category
Live-Dead Cell Staining
Kit's other name
Cell viability assay kit
Contents
Assay Buffer Live Cell Staining Dye Dead Cell Staining Dye
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Mammalian
Applications
• Screening/studying/characterization of stimulators/inhibitors that affect cell viability.
Samples tested
·    Adherent cells or suspension cells
Tissue
cell, cells
Test
Biovision supplies other types of Assays as 1.
Description
For cells, cell lines and tissues in culture till half confluency.