EZCell™ Glutathione Detection Kit (Blue Fluorescence)

Glutathione (GSH) represents major low molecular-weight free thiol in living cells. As the key antioxidant in mammalian cells, intracellular GSH forms rapidly eliminated conjugates with electrophilic xenobiotics, free radicals as well as hydroperoxides. Insufficiency of cellular GSH results in oxidative stress damage and mitochondrial degeneration. Diminished levels of glutathione are symptomatic of cell stress thus; it is advantageous to monitor distribution of the intracellular GSH as an indicator of early apoptosis and cell death. BioVision’s EZCellTM Glutathione Detection Kit (Blue Fluorescence) utilizes Monochlorobimane (MCB), a non-fluorescent and cell-permeable dye, that forms highly fluorescent adducts with GSH (GSH-MCB) detectable at EX/EM= 380/465 ±20 nm respectively. The reaction is catalyzed by glutathione-S-transferase (GST) and the intensity of the fluorescence reflects the amount of intracellular GSH. Hence, our kit provides a useful tool for fast and easy evaluation of the GSH effectors directly in the living cells.

• Detection method : Fluorescence (Ex/Em = 380/465 nm) • Applications: • Rapid and sensitive detection of intracellular levels of GSH and activity of GST in live cells • Screening for compounds affecting intracellular GSH levels

• Highly sensitive fluorometric method to measure intra-cellular Glutathione levels in response to a variety of biochemical stimuli • Simple & High throughput-adaptable • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect Glutathione metabolism

cell based assays, metabolism

none

• Wash Buffer • Monochlorobimane (MCB)

-20°C

gel pack

12 months

see datasheet

• Rapid and sensitive detection of intracellular levels of GSH and activity of GST in live cells • Screening for compounds affecting intracellular GSH levels

Live cell cultures (adherent or suspension)

Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.

Blue Substrate

The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.

Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.