Cobalt Colorimetric Assay Kit

Cobalt is a hard, lustrous, grey metal, a chemical element (symbol Co, atomic number 27 and atomic mass 58.9). Cobalt is an essential trace-element for all multicellular organisms as the active center of coenzymes called cobalamins. These include vitamin B-12 which is essential for plants and animals [1]. Cobalt is also an active nutrient for bacteria, algae, and fungi, and may be a necessary nutrient for all life. Cobalt, particularly the Co²ᶧ species, forms complexes with a large number of organic and inorganic molecules with a range of extinction coefficients in the visible range. The BioVision Cobalt Assay kit takes advantage of the reaction of Co²ᶧ with mercaptoethanol in basic media. Under the condition, Cobalt forms a complex with a strong absorbance band at 475 nm. The metal ions Fe²ᶧ, Cu²ᶧ, Ni²ᶧ, Zn²ᶧ, Mn²ᶧ, exhibit less than 10% interference at this wavelength. The kit provides a quick simple accurate method of quantitating Co²ᶧ in a variety of samples.

• Detection method: Absorbance (475 nm) • Species reactivity: Mammalian • Applications: The assay is linear in the range 3 to 50 nmol (0.18:3 µg) Cobalt, with a detection limit of ~ 3 nmol (or ~15 µM, 0.18 µg).

• Simple procedure; takes only 20 minutes • Fast and convenient • The BioVision Cobalt Assay kit takes advantage of the reaction of Co²ᶧ with mercaptoethanol in basic media.

none

• Cobalt Reagent • Cobalt Chloride Standard (1.0 µmol)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.