Bacterial Counting Colorimetric Assay Kit

Catalog number
K511-2500
Name
Bacterial Counting Colorimetric Assay Kit
Size
2500 assays
Price
732.00 EUR
Supplier

Details

Kit's description
The Bacteria Counting Colorimetric Assay Kit is a simple and sensitive assay to quantify bacterial concentration and viability. The assay is based on the cleavage of a water soluble tetrazolium salt (WST) to formazan by mitochondrial dehydrogenases. The color produced is directly proportional to the number of viable bacteria and can be quantified using a microplate reader (Absorbance OD 460 nm). The assay is sensitive and can detect the growth of low density cultures starting with as few as 10 bacteria per well at the time of adding the reagent. The assay can also be used to count the number of living bacteria in a broth culture or to study bacterial viability in response to inhibitors, media types, antibiotics, growth and heterologous protein overexpression conditions.
Highlights
• Detection method: Absorbance (460) • Species reactivity: Bacteria • Applications: The assay is a simple method to quantify bacteria concentration and viability. Can detect bacterial growth as few as 10 bacteria per sample
Kit's benefits
• Simple procedure; takes less than ~90 minutes • Fast and convenient
Category
Other Metabolism-related assays
Kit's other name
none
Contents
• Electrocoupling Solution (ECS) • WST Reagent (lyophilized)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Bacteria
Applications
The assay is a simple method to quantify bacteria concentration and viability. Can detect bacterial growth as few as 10 bacteria per sample
Samples tested
E. coli, Gram positive and Gram negative bacteria
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.