Chitotriosidase Activity Assay Kit (Fluorometric)

Chitotriosidase (CHIT1 or chitinase-1), is a mammalian enzyme belonging to glycosyl hydrolase-18 family (EC 3.2.1.14), which can hydrolyze the β-(1, 4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. CHIT1 is synthesized exclusively by activated macrophages both in normal and inflammatory conditions and, as a host defense factor, CHIT1 plays a pivotal role in infectious disease such as malaria and fungi infectious. Increased CHIT1 activity has also been found in patients suffering diseases such as bronchial asthma and atherosclerosis. CHIT1 has been used as a valuable diagnostic biomarker for monitoring the therapeutic efficacy of treatments for Gaucher’s disease or β-glucocerebrosidase deficiency. BioVision’s Chitotriosidase Activity Assay, utilizes a fluorogenic substrate that can be hydrolyzed by chitinase and a set of proprietary assay buffers that can distinguish specific CHIT1 activity from other hydrolases including Acidic Mammalian Chitinase. The kit provides a simple, specific, sensitive assay that can detect as low as 0.2 mU/ml of CHIT1 in a variety of biological samples.

• Detection method: Fluorescence (Ex/Em 320/445 nm) • Applications: Measurement of Chitotriosidase activity in various biological samples and purified/crude enzyme preparations

• Simple, rapid & convenient assay to measure Chitotriosidase activity • Includes Positive Control (Chitotriosidase)

Carbohydrate Metabolism

none

• CHIT1 Assay Buffer • CHIT1 Inhibition Buffer • CHIT1 Substrate (in DMSO) • Chitotriosidase (lyophilized) • 4-Methylumbelliferone Standard (5 mM)

-20°C

gel pack

12 months

Mammalian

Measurement of Chitotriosidase activity in various biological samples and purified/crude enzyme preparations

• Adherent and suspension cell lysates • Animal tissues such as rat liver • Purified enzyme

Biovision supplies other types of Assays as 1.