Acidic Mammalian Chitinase Activity Kit (Fluorometric)

Catalog number
K513-100
Name
Acidic Mammalian Chitinase Activity Kit (Fluorometric)
Size
100 assays
Price
732.00 EUR
Supplier

Details

Kit's description
Acidic Mammalian Chitinase (AMCase) and Chitotriosidase-1(CHIT1) are the only two mammalian active chitinases that belong to the glycosyl hydrolase-18 family (EC 3.2.1.14). They are chitin-fragmenting hydrolases that hydrolyze the β-(1,4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. In contrast to CHIT1, AMCase exhibits its optimum activity under acidic conditions and is relatively abundant in the gastrointestinal tract and lungs. AMCase is found highly expressed both in animal models used for the study of allergic inflammation and in lungs of human asthma. Recent data suggests a major biological function of AMCase in asthma and the changes in AMCase expression can be used to monitor Th-2/IL-13-mediated immune response and inflammation. BioVision’s Acidic Mammalian Chitinase Activity Assay Kit utilizes the ability of AMCase to cleave a synthetic substrate to release the free fluorophore which can be easily quantified (Ex/Em= 320/445 nm) and it uses a specific Lysis Buffer which distinguishes AMCase activity from CHIT1 activity. This kit provides a simple, specific, ultra-sensitive assay that can detect as low as 0.5 mU/ml of AMCase activity in a variety of biological samples
Highlights
• Detection method: Fluorescence (Ex/Em 320/445 nm) • Applications: Measurement of Chitinase activity in various biological samples and purified/crude enzyme preparations
Kit's benefits
• Simple, rapid & convenient assay to measure Acidic Mammalian Chitinase • Includes Positive Control (Acidic Mammalian Chitinase)
Category
Carbohydrate Metabolism
Kit's other name
none
Contents
• AMCase Assay Buffer • AMCase Lysis Buffer • AMCase Substrate (in DMSO) • Acidic Mammalian Chitinase (lyophilized) • 4-Methylumbelliferone Standard (5 mM)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Mammalian
Applications
Measurement of Chitinase activity in various biological samples and purified/crude enzyme preparations
Samples tested
• Adherent and suspension cell lysates • Animal tissues such as rat liver • Purified enzyme