Methyltransferase Activity Assay Kit (Fluorometric)

Catalog number
K521-100
Name
Methyltransferase Activity Assay Kit (Fluorometric)
Size
100 assays
Price
682.00 EUR
Supplier

Details

Kit's description
Methyltransferases are a diverse group of enzymes that function to methylate protein, DNA, and small molecule targets. Accordingly, methyl transferase activity plays a critical role in modulating gene transcription, protein interactions, and signaling networks. Anomalous DNA methylation pattern has been consistently detected during cancer development as well as in many genetic disorders such as Fragile X Syndrome, ICF, and Rett Syndrome. BioVision’s Methyltransferase Activity Fluorimetric Assay Kit allows for kinetic evaluation of methyltransferase activity of purified enzymes. The transfer of a methyl group from S-Adenosyl Methionine (SAM) cofactor to a corresponding substrate generates S-Adenosyl Homocysteine (SAH) product. SAH is detected by coupling the methyl transfer reaction to a multi-step enzymatic cascade, resulting in the generation of an intermediate that reacts with OxiRedTM Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. The limit of quantification (L.O.Q) is 7.5 pmol of SAH generated per min per ml (7.5 µU/ml) in purified enzymes.
Highlights
• Detection method: Fluorometric (Ex/Em 535/587 nm) • Applications: Measurement of Methyltransferase activity in purified/crude enzyme preparations and analysis of methyltransferase inhibitors
Kit's benefits
• Simple, rapid & convenient assay to measure Methyltransferase Activity in purified enzyme • Includes Positive Control
Category
Metabolism
Kit's other name
none
Contents
• MT Assay Buffer • Enzyme Re-suspension Buffer • Enzyme Mix I • Enzyme Mix II • Enzyme Mix III • SAM Cofactor (0.5 mM) • SAH Standard (100 μM) • MT Positive Control • OxiRedTM Probe
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Mammalian
Applications
Measurement of Methyltransferase activity in purified/crude enzyme preparations and analysis of methyltransferase inhibitors
Samples tested
Purified enzyme
Test
Biovision supplies other types of Assays as 1.