Plasmid Miniprep Kit

Amplification and purification of plasmid DNA is a necessary step for numerous recombinant DNA technology applications. BioVision’s Plasmid Miniprep Kit eases the purification of low and high copy plasmids from bacteria. DNA purified by this kit can be used for a variety downstream molecular biology applications such as cloning, PCR, transfections/transformations, sequencing, enzymatic analysis etc

• Useful for PCR, transformations, transfections, restriction enzyme digest/analysis, sequencing

DNA

none

• Buffer R [Re-suspension Buffer] • RNAse A • Buffer L [Lysis Buffer] • Buffer N [Neutralization Buffer] • Buffer ER [Endonuclease Removal Buffer] • Buffer W [Wash Buffer] • Buffer E [Elution Buffer] • Spin Columns/Collection Tubes

-20°C/RT

gel pack

24 months

Bacterial

Useful for PCR, transformations, transfections, restriction enzyme digest/analysis, sequencing

Isolation of low and high copy plasmids from bacteria

Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.