Cyclooxygenase-1 (COX-1) Inhibitor Screening Kit (Fluorometric)

Cyclooxygenase (COX), also known as prostaglandin-endoperoxide synthase (PTGS, EC 1.14.99.1), is an enzyme that is responsible for the formation of important biological mediators called prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is the central enzyme in the biosynthetic pathway to prostanoids from arachidonic acid. There are two known isoenzymes: COX-1 and COX-2. COX-1 is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in kidney. COX-2 is not expressed under normal conditions in most cells, but elevated levels are found during inflammation. Pharmacological inhibition of COX by non-steroidal anti-inflammatory drugs (NSAID) can provide relief from the symptoms of inflammation and pain. BioVision’s COX-1 Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of COX-1 inhibitors. The assay is based on the fluorometric detection of Prostaglandin G2, the intermediate product generated by the COX enzyme.

• Detection method: Fluorescence (Ex/Em 535/587 nm) • Applications: Screening/studying/characterizing COX:1 inhibitors.

• Simple method to screen COX-1 inhibitors • High-throughput • Includes Inhibitor Control, SC560

Signal Transduction, COX-1

Prostaglandin-Endoperoxide Synthase 1 Inhibitor Screening Kit (Fluorometric), PTGS1 Inhibitor Screening Kit (Fluorometric)

• COX Assay Buffer • COX Probe (in DMSO) • COX Cofactor (in DMSO) • Arachidonic Acid • NaOH • COX-1, Ovine • SC560, COX-1 inhibitor (in DMSO)

-20°C

gel pack

12 months

see datasheet

Screening/studying/characterizing COX-1 inhibitors.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.