Urine Albumin-to-Creatinine Ratio (UACR) Assay Kit

Urine Albumin-to-Creatinine Ratio (UACR) is one of the two markers used to determine chronic kidney disease (CKD). UACR is recommended to be measured on regular basis on people living with Type I and Type II diabetes. UACR is defined as the ratio between albumin (reported in mg/dl) and creatinine (reported in g/dl). This ratio estimates the amount of albumin excreted in urine during a 24 hr period. Albuminuria is diagnosed when UACR is greater than 30 mg albumin/g creatinine. BioVision’s UACR Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects albumin (detection range: 0.02- 2.5 mg/ml), creatinine (detection range: 0.002 -0.5 mg/ml) and urine albumin-to-creatinine ratio. The UACR ratio is determined in two steps: First, albumin is determined by using a probe (AB580) that specifically recognizes albumin (Ex/Em = 600/630 nm). Second, creatinine is converted to sarcosine via enzymatic reactions. Sarcosine is specifically oxidized generating a product that reacts with a probe producing a chromophore that can be detected at 570 nm.

• Detection method: Fluorescence (Ex/Em = 600/630 nm) & Absorbance (OD 570 nm) • Applications: Estimation of albumin in biological samples : Estimation of creatinine in biological samples : Determination of UACR in mammalian urine samples

• Simple & Sensitive • High-Throughput adaptable • Suitable for detecting albumin (detection range: 0.02- 2.5 mg/ml), creatinine (detection range: 0.002 -0.5 mg/ml) and urine albumin-to-creatinine ratio (UACR)

Metabolism, Urine Albumin-to-Creatinine Ratio, UACR

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• Creatinine Assay Buffer • Albumin Assay Buffer • Albumin Diluent • Albumin Probe (AB580) • Creatinine Probe • Creatinase • Creatininase • Creatinine Enzyme Mix • BSA Standard (2 mg/ml) • Creatinine Standard (10 μmol)

-20°C

gel pack

12 months

see datasheet

• Estimation of albumin in biological samples • Estimation of creatinine in biological samples • Determination of UACR in mammalian urine samples

• Albumin: urine, saliva, etc. • Creatinine: urine, serum, etc. • UACR: urine

Biovision supplies other types of Assays as 1.