Sphingomyelinase Activity Fluorometric Assay Kit

Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation and sterol homeostasis. BioVision’s SMase Fluorometric Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using fluorescence (Ex/Em = 535/587 nm). Neutral sphingomyelinase (N-SMase) is a Mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce fluorescence. This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 10 µU/ml in a variety of samples.

• Detection method: Fluorescence (Ex/Em 535/587 nm) • Applications: Measurement of SMase activity in various tissues/cells extracts

• Simple, rapid & convenient • The assay can detect sphingomyleinase activity as low as 10 µU/ml in a variety of samples.

Sphingomyleinase, Sphingomyleinase activity

none

• SMase Assay Buffer • SMase Probe (DMSO) • SMase Substrate (Lyophilized) • SMase Enzyme Mix I (Lyophilized) • SMase Enzyme Mix II (Lyophilized) • Choline Standard (Lyophilized) • SMase Positive Control (Lyophilized) • SMase Extraction Detergent

-20°C

gel pack

12 months

see datasheet

Measurement of SMase activity in various tissues/cells extracts

• Animal tissues: brain, heart, kidney, etc. • Cell culture: Adherent or suspension cells

Biovision supplies other types of Assays as 1.