Tyrosinase Inhibitor Screening Kit (Colorimetric)

Tyrosinase or polyphenol oxidase (EC 1.14.18.1), is an oxidoreductase that participates in the biosynthesis of melanin, a ubiquitous biological pigment found in hair, eyes, skin, etc. Inhibition of tyrosinase has been a long-time target in the skin health research, cosmetics and agricultural industries because of its role in browning reactions in skin pigmentation and during fruit harvesting and handling. Skin whitening and bleaching products utilize natural or synthetic tyrosinase inhibitors in order to lighten the skin color. Polyphenols, benzaldehyde derivatives, long-chain lipids, steroids, and natural compounds have been used as tyrosinase inhibitors. Tyrosinase catalyzes the oxidation of tyrosine, producing a chromophore that can be detected at 510 nm. In the presence of Kojic Acid, a reversible inhibitor of tyrosinase, the rate of oxidation of the substrate is decreased. The kit provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of tyrosinase inhibitors. The assay is also adaptable to a 384-well format.

• Detection method: Absorbance (OD 510 nm) • Applications: Screening/studying/characterizing tyrosinase inhibitors

• Rapid, simple & sensitive • Convenient & HTS • Includes inhibitor control Kojic acid

Tyrosinase Inhibitor

none

• Tyrosinase Assay Buffer • Tyrosinase Substrate • Tyrosinase (Lyophilized) • Tyrosinase Enhancer • Inhibitor Control (Kojic Acid)

-20°C

gel pack

12 months

see datasheet

Screening/studying/characterizing tyrosinase inhibitors

Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.