Phosphatidylcholine Colorimetric/Fluorometric Assay Kit

Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid. BioVision's Phosphatidylcholine Assay Kit is a simple convenient means of measuring Phosphatidylcholine in a variety of biological samples. The kit utilizes an enzyme-coupled assay in which PC is hydrolyzed, releasing choline which is subsequently oxidized resulting in development of the OxiRed probe to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). BioVision’s Phosphatidylcholine kit measures PC in the range of 0.1 to 10 nmol per sample. PC is present in serum at ~ 0.2-2.5 mM (~50-200 mg/dL).

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: BioVision’s Phosphatidylcholine kit measures PC in the range of 0.1 to 10 nmol per sample.

• Simple procedure; takes less than ~40 minutes • Fast and convenient

none

• PC Assay Buffer • OxiRed Probe • PC Hydrolysis Enzyme • PC Development Mix • PC Standard (10 µmol)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.