Cholesterol Efflux Fluorometric Assay Kit (cell-based)

Cholesterol efflux from the peripheral tissues and cells in atherosclerotic plaque is an initial and critical step in Reverse Cholesterol Transport (RCT). RCT is the process by which extrahepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, transport excessive cholesterol back to the liver for bile acid synthesis and excretion, thus lowering the peripheral lipid burden. A negative correlation has been established between the in vitro efflux of cholesterol from macrophages and atherosclerosis. BioVision’s Cholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides a safe, sensitive, and reproducible method for measuring cholesterol efflux.

• Detection method: Fluorescence (Ex/Em = 482/515 nm). • Applications:• Screen serum samples or lipoproteins (isolated or recombinant) for cholesterol efflux. • Screen small molecules for their effect on cholesterol efflux (a valuable tool for drug discovery program).

• Simple & Rapid Protocol • Convenient: Non-Radioactive, no special handling or disposal required • High-Throughput • Accurate: reproducible results with low intra & inter assay variability

Cholesterol

none

• Labeling Reagent • Equilibration Buffer • Reagent A • Reagent B • Cell Lysis Buffer • Positive Control • Serum Treatment Reagent

-20°C

gel pack

12 months

see datasheet

• Screen serum samples or lipoproteins (isolated or recombinant) for cholesterol efflux. • Screen small molecules for their effect on cholesterol efflux (a valuable tool for drug discovery program).

Serum, Isolated, or recombinant lipoproteins

cell

Biovision supplies other types of Assays as 1.

For cells, cell lines and tissues in culture till half confluency.