HMG-CoA Reductase Activity/Inhibitor Screening Kit (Colorimetric)

HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is the rate-controlling enzyme of the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. The enzyme is target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol. BioVision’s HMG-CoA Reductase Activity Assay Kit measures utilization of NADPH, which can be measured by the decrease of absorbance (OD 340 nm). The kit is suitable for measuring activity of purified enzyme and screening inhibitors/activators of HMG-CoA reductase. The limit of detection is below 0.05 mU.

• Detection method: Absorbance (OD 340 nm) • Applications: Measurement of HMG:CoA reductase activity : Screening/studying/characterizing HMG:CoA reductase inhibitors/activators

• Simple, rapid & convenient • Limit of detection: below 0.05 mU • Includes inhibitor (Atorvastatin)

Lipid Metabolism

HMGR Activity/Inhibitor Screening Kit (Colorimetric), 3-hydroxy-3-methyl-glutaryl-CoA reductase Activity/Inhibitor Screening Kit (Colorimetric)

• HMG-CoA Reductase Assay Buffer • HMG-CoA Reductase • HMG-CoA • NADPH • Inhibitor (Atorvastatin, 10 mM)

-20°C

gel pack

12 months

see datasheet

• Measurement of HMG-CoA reductase activity • Screening/studying/characterizing HMG-CoA reductase inhibitors/activators

• Partially purified and recombinant enzyme

Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.