Succinyl-CoA Synthetase Activity Colorimetric Assay Kit

Succinyl-CoA Synthetase (SCS, also called Succinyl-CoA ligase, Succinate Thiokinase) (EC 6.2.1.5) is a critical enzyme in the citric acid cycle and an important metabolic intermediate for porphyrin, heme and ketone body biosynthesis. It is located in the mitochondrial matrix and is a heterodimer composed of one α and one β subunit. In humans, Succinyl-CoA Synthetase deficiency causes the build-up of lactic acid leading to lactic acidosis, which can be fatal in infants. Measurement and analysis of SCS activity is useful for both mechanistic studies as well as for diagnostic purposes. In BioVision’s Succinyl-CoA Synthetase Activity Assay, SCS converts succinate into succinyl-CoA in the presence of ATP and CoA. Succinyl-CoA reacts with the Developer to form a colored product with strong absorbance at 450 nm. This assay kit is simple, sensitive, and high-throughput adaptable. It can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples.

• Detection method: Absorbance (OD 450 nm). • Applications: • Measurement of Succinyl:CoA Synthetase activity in various tissues/cells • Analysis of cell signaling pathway

• Simple & Rapid Protocol • High-Throughput adaptable • This kit can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples.

Succinyl-CoA Synthetase

none

• SCS Assay Buffer • SCS Substrate Mix (Lyophilized) • SCS Enzyme Mix (Lyophilized) • SCS Developer (Lyophilized) • NADH Standard (Lyophilized) • SCS Positive Control (Lyophilized)

-20°C

gel pack

12 months

see datasheet

• Measurement of Succinyl-CoA Synthetase activity in various tissues/cells • Analysis of cell signaling pathway

• Animal tissues: heart, liver, muscle, etc. • Purified mitochondria • Cell culture: adherent or suspension cells

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.