Acid Sphingomyelinase Activity Colorimetric Assay Kit

Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision’s Acid Sphingomyleinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.

• Detection method: Absorbance (570 nm) • Applications : Measurement of ASMase activity in various tissues/cell extracts : Screening of ASMase inhibitors

• Simple & Rapid Protocol • High-Throughput adaptable • This kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.

Acid sphingomyleinase

none

• ASMase Assay Buffer I • ASMase Assay Buffer II • ASMase Substrate (Lyophilized) • ASMase Enzyme Mix I (Lyophilized) • ASMase Enzyme Mix II (Lyophilized) • Choline Standard (Lyophilized) • ASMase Positive Control (Lyophilized) • ASMase Probe (DMSO)

-80°C

gel pack

6 months

see datasheet

• Measurement of ASMase activity in various tissues/cell extracts • Screening of ASMase inhibitors

• Animal tissues: brain, heart, kidney, etc. • Cell culture: Adherent or suspension cells • Serum

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.