Acid Sphingomyelinase Activity Colorimetric Assay Kit

Catalog number
K598-100
Name
Acid Sphingomyelinase Activity Colorimetric Assay Kit
Size
100 assays
Price
601.00 EUR
Supplier

Details

Kit's description
Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision’s Acid Sphingomyleinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.
Highlights
• Detection method: Absorbance (570 nm) • Applications : Measurement of ASMase activity in various tissues/cell extracts : Screening of ASMase inhibitors
Kit's benefits
• Simple & Rapid Protocol • High-Throughput adaptable • This kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.
Category
Acid sphingomyleinase
Kit's other name
none
Contents
• ASMase Assay Buffer I • ASMase Assay Buffer II • ASMase Substrate (Lyophilized) • ASMase Enzyme Mix I (Lyophilized) • ASMase Enzyme Mix II (Lyophilized) • Choline Standard (Lyophilized) • ASMase Positive Control (Lyophilized) • ASMase Probe (DMSO)
Storage condition
-80°C
Shipping condition
gel pack
Maximum time can be stored
6 months
Species reactivity
see datasheet
Applications
• Measurement of ASMase activity in various tissues/cell extracts • Screening of ASMase inhibitors
Samples tested
• Animal tissues: brain, heart, kidney, etc. • Cell culture: Adherent or suspension cells • Serum
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.