Sphingomyelin Quantification Colorimetric Assay Kit

Sphingomyelin is an important structural lipid component of cell membranes and lipoproteins. Sphingomyelin has been implicated in the pathogenesis of several diseases; therefore a sensitive and reliable technique for its quantification is of considerable importance. BioVision’s Sphingomyelin Quantification Assay Kit provides a simple and sensitive method for quantifying sphingomyelin using colorimetry. In the assay, sphingomyelin is hydrolyzed into ceramide and phosphorylcholine by sphingomyelinase. Alkaline phosphatase (ALP) dephosphorylates phosphorylcholine to choline, which then reacts with Sphingomyelin Enzyme Mix to produce an intermediate. The intermediate reacts with a highly specific probe to generate color (OD 570 nm). The kit can detect sphingomyelin in various biological samples as low as 1 µM.

• Detection method: Absorbance (OD 570 nm) • Applications: : Sphingomyelin quantification in various biological samples : Analysis of cell signaling pathway

• Simple, rapid & convenient • The assay can detect sphingomylein less than 1 µM in a variety of samples

Sphingomylein

none

• SM Assay Buffer • OxiRed Probe™ • Sphingomyelinase (Lyophilized) • ALP Enzyme (Lyophilized) • SM Enzyme Mix (Lyophilized) • SM Standard (5 mM)

-20°C

gel pack

12 months

see datasheet

• Sphingomyelin quantification in various biological samples • Analysis of cell signaling pathway

• Serum, plasma • Tissue samples such as brain etc. • Adhesion or suspension cells

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.