Lactate Colorimetric/Fluorometric Assay Kit

Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate. In the Lactate Assay Kit, lactate specifically reacts with a enzyme mix to generate a product, which interacts with lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm). The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need of pretreatment or purification of samples. The kit can detect 0.001-10 mM of various Lactate samples.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The kit can detect 0.001:10 mM of various Lactate samples.

• Simple procedure; takes ~40 minutes • Fast and convenient • Kit contains the necessary reagents for accurate measurement of Lactate colorimetrically and fluorometrically

Lactate Assay

none

• Lactate Assay Buffer • Lactate Probe (in DMSO) • Lactate Enzyme Mix • L(+)-Lactate Standard (100 nmol/µl)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.