Pyruvate Colorimetric/Fluorometric Assay Kit

Pyruvate is a central molecule in metabolism through which sugars enter the citric acid cycle. Pyruvate can be converted to carbohydrates during gluconeogenesis or to fatty acids via acetyl CoA. High levels of pyruvate are associated with liver disease and genetic disorders. Pyruvate has also been used to stimulate metabolism leading to loss of body weight. BioVision provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc. In the assay, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate color (λ= 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvate content, the pyruvate concentration can be accurately measured. The kit detects 1 µM to 10 mM pyruvate.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Application: The kit detects 1 µM to 10 mM pyruvate.

• Simple procedure; takes ~40 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of pyruvate concentrations in biological samples

Pyruvate Assay

none

• Pyruvate Assay Buffer • Pyruvate Probe (in DMSO) • Pyruvate Enzyme Mix • Pyruvate Standard (100 nmol/µl)

-20°C

gel pack

12 months

see datasheet

Inquire

• Animal tissues • Cell culture: adherent or suspension cells • Serum, saliva

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.