Adipogenesis Colorimetric/Fluorometric Assay Kit

Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured. BioVision’s adipogenesis assay kit quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect triglyceride in as few as a thousand or less differentiated 3T3-L1 cells with triglyceride detection linear range 0.2 to 10 nmol. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or inhibitors, or to screen drugs.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Application: The kit can detect triglyceride in as few as a thousand or less differentiated 3T3:L1 cells with triglyceride detection linear range 0.2 to 10 nmol.

• Simple procedure; takes ~40 minutes • Fast and convenient • Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipogenesis in various samples.

Obesity

none

• Adipogenesis Assay Buffer • Lipid Extraction Solution • Adipogenesis Probe (in DMSO solution) • Lipase (lyophilized) • Adipogenesis Enzyme Mix (lyophilized) • Triglyceride Standard (1 mM)

-20°C

gel pack

12 months

see datasheet

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Adipocyte precursors such as 3T3 cells, human preadipocytes etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.