Free Fatty Acid Quantification Colorimetric/Fluorometric Kit

Fatty Acids play very important roles in normal metabolism and many disease developments. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. BioVision’s Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In the assay, Fatty Acids are converted to their CoA derivatives, which are subsequently oxidized with the concomitant generation of color or fluorescence. C-8 (octanoate) and longer fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (at Ex/Em = 535/587 nm) methods with detection limit 2 µM free fatty acid in variety samples.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Application: The kit has a detection limit 2 µM free fatty acid in variety samples.

• Simple procedure; takes ~40 minutes • Fast and convenient • Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipolysis in cell culture samples

Fatty Acid Assay

none

• Fatty Acid Assay Buffer • Fatty Acid Probe (in DMSO) • ACS Reagent • Enzyme Mix • Enhancer • Palmitic Acid Standard (1nmol/µl)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids

Colorimetric assays or detection use UV absorption or enzymatic color reaction.