PicoProbe™ Triglyceride (TG) Fluorometric Assay Kit

Triglycerides (TG) are the main constituents of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as an energy source. TG is broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis. Biovision’s PicoProbeTM Triglyceride Assay kit is suitable for measuring triglyceride levels in samples, which contain reducing substances that may interfere with oxidase-based assays. In this assay, TG is hydrolyzed to glycerol and fatty acid. The glycerol reacts with Triglyceride Enzyme Mix to form an intermediate product, which in turn reacts with PicoProbe & Developer to generate the fluorescence. The generated fluorescence is directly proportional to the amount of triglycerides. This high-throughput adaptable assay kit is simple, sensitive and easy to use & can detect less than 0.4 µM triglycerides in a variety of samples.

• Detection method: Fluorescence (Ex/Em 535/587 nm) • Applications: Measurement of triglycerides in various tissues, cells & other biological fluids. Analysis of lipid metabolism. Mechanistic study of cardiovascular disease.

• Simple, sensitive, easy to use, and high-throughput adaptable. • Kit contains all necessary reagents for accurate measurement of triclyceride levels. • This kit can detect less than 0.4 µM triglycerides in a variety of samples.

Triglyceride Assay

Triacylglycerol Assay Kit, Triacylglyceride Assay Kit, TAG Assay Kit

• Triglyceride Assay Buffer • PicoProbe™ (in DMSO) • Lipase (lyophilized) • Triglyceride Enzyme Mix (lyophilized) • Triglyceride Developer (lyophilized) • Triglyceride Standard (1 mM)

-20°C

gel pack

12 months

see datasheet

Inquire

• Animal tissues: Liver, pancreas, heart etc. • Cell culture: adipocytes etc. • Biological fluids: serum, plasma & saliva.

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit