Ethanol Colorimetric/Fluorometric Assay Kit

Alcohol (ethanol C₂H₅OH) is among the most widely consumed drinks. Low doses of alcohol may help circulation while heavy alcohol consumption may lead to various forms of disease. Quantitative determination of alcohol finds applications in basic research, drug discovery, clinical studies and fermentation industry processes. BioVision’s Ethanol Assay Kit provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media. Alcohol oxidase oxidizes ethanol to generate H₂O₂ which reacts with our probe to generate color (λmax= 570 nm) and fluorescence (Ex/Em = 535/587 nm).. The kit detects 0.1-10 ppm alcohol (~10-800 nM).

• Detection method:Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Sample type: Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids • Species reactivity: Mammalian • Application: The kit detects 0.1:10 ppm alcohol (~10:800 nM).

• Simple procedure; takes ~40 minutes - 1 hour • Fast and convenient • Kit contains the necessary reagents for accurate measurement of ethanol in biological samples

Ethanol Assay

none

• Ethanol Assay Buffer • Ethanol Probe (in DMSO,anhydrous) • Ethanol Enzyme Mix (lyophilized) • Ethanol Standard (MW:46.07, 17.15N)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.