Lactose Colorimetric/Fluorometric Assay Kit

Lactose (C₁₂H₂₂O₁₁ FW: 342.3) is an important naturally occurred disaccharide, consisting of one galactose and one glucose. Milk contains ~2-8% lactose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage. In the BioVision Lactose Assay Kit, Lactose is hydrolyzed to glucose and galactose. The galactose is subsequently oxidized generating color (O.D 570 nm) and fluorescence (Ex/Em 535/587 nm). Free galactose can be corrected by a background control in the absence of lactase. The Lactose Assay Kit provides a simple, convenient, and sensitive means for direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). Pretreatment of samples is not required. The kit can be used as a high throughput assay.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian

• Simple procedure; takes ~1 hour • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Lactose

Lactose

none

• Lactose Assay Buffer • Probe (DMSO solution) • Lactase (Lyophilized) • Lactose Enzyme Mix (Lyophilized) • HRP (Lyophilized) • Lactose Standard (100 nmol/µl)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, monitoring level during fermentation and feeding in protein expression processes nad growth medium

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.