Creatinine Colorimetric/Fluorometric Assay Kit

Creatinine is a breakdown product of creatine phosphate. Creatinine is produced and excreted at a constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function. Blood creatinine levels increase only in cases of significant (>75%) damage to nephrons. Creatinine clearance is frequently used as a means of standardizing excretion of other compounds such as isoprostanes. BioVision’s Creatinine Assay Kit provides an accurate, convenient measure of creatine concentration in biological fluids such as serum, urine or CSF. In the assay, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 538/587 nm). Unlike the picric acid assay, this kit is suitable for serum/plasma creatinine determinations, as well as for urine and other biological samples.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian

• Simple procedure; takes ~1 hour • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Creatinine

Creatinine

none

• Creatinine Assay Buffer • Creatinine Probe • Creatinase • Creatininase • Creatinine Enzyme Mix • Creatinine (10 µmol)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.