Lactate Colorimetric Assay Kit II

Lactate (CH₃CH(OH)COO-) plays important roles in many biological processes. Abnormally high concentrations of lactate have been related to disease states such as diabetes and lactic acidosis, etc. L(+)-Lactate is the major lactate stereoisomer formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate. In the Lactate Assay Kit, lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (λmax = 450 nm). The kit detect L(+)-Lactate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples. It detects 0.02 mM – 10 mM lactate in various samples.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: Kit detects 0.02 mM – 10 mM lactate in various samples.

• Simple procedure; takes ~40 minutes • Fast and convenient • Kit contains the necessary reagents for accurate measurement of Lactate

Lactate Assay

none

• Lactate Assay Buffer • Lactate Enzyme Mix • Lactate Substrate Mix • L(+)-Lactate Standard (100 mM)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, monitoring level during fermentation and feeding in protein expression processes

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.